Identification of a cDNA from the Arbuscular Mycorrhizal Fungus Glomus intraradices that is Expressed During Mycorrhizal Symbiosis and Up-Regulated by N Fertilization

Identification of a cDNA from the Arbuscular Mycorrhizal Fungus Glomus intraradices that is Expressed During Mycorrhizal Symbiosis and Up-Regulated by N Fertilization

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A cDNA library was constructed with RNA from Glomus intraradices-colonized lettuce roots and bostik universal primer pro used for differential screening.This allowed the identification of a cDNA (Gi-1) that was expressed only in mycorrhizal roots and was of fungal origin.The function of the gene product is unknown, because Gi-1 contained a complete open reading frame that was predicted to encode a protein of 157 amino acids which only showed little homology with glutamine synthetase from Helicobacter pylori.

The time-course analysis of gene expression during the fungal life cycle showed that Gi-1 was expressed only during the mycorrhizal symbiosis and was not detected in dormant or germinating spores of G.intraradices.P fertilization did not significantly change the pattern of Gi-1 expression compared with that in the unfertilized treatment, whereas N fertilization (alone or in combination with P) considerably enhanced the Gi-1 transcript accumulation.

This increase in gene bekindtopets.com expression correlated with plant N status and growth under such conditions.The possible role of the Gi-1 gene product in intermediary N metabolism of arbuscular mycorrhizal symbiosis is further discussed.